V-ATPase

Vacuolar type H+-ATPase (V-ATPase) is a highly conserved evolutionarily ancient enzyme with remarkably diverse functions in eukaryotic organisms. V-ATPases acidifiy a wide array of intracellular organelles and pump protons across the plasma membranes of numerous cell types. V-ATPases couple the energy of ATP hydrolysis to proton transport across intracellular and plasma membranes of eukaryotic cells.


V-ATPase schematic

Roles played by V-ATPases

V-ATPases are found within the membranes of many organelles, such as endosomes, lysosomes and secretory vesicles where they play a variety of roles crucial for the function of these organelles. For example, the proton gradient across the yeast vacuolar membrane generated by V-ATPases drives calcium uptake into the vacuole through an H+/Ca++ antiporter system. V-ATPases also play an important role in synaptic transmission in neuronal cells. Norepinephrine enters vesicles in exhange for protons pumped by V-ATPase.

V-ATPases are also found in the plasma membranes of a wide variety of cells such as intercalated cells of the kidney, osteoclasts (bone resorbing cells), macrophages, neutrophils, sperm, midgut cells of insects and certain tumor cells. Plasma membrane V-ATPases are involved in processes such as pH homeostasis, coupled transport and tumor metastasis. V-ATPases in the acrosomal membrane of sperm acidify the acrosome. This acidification activates proteases required to drill through the plasma membrane of the egg. V-ATPases in the osteoclast plasma membrane pump protons onto the bone surface which is necessary for bone resorption. In the intercalated cells of the kidney, V-ATPases pump protons into the urine, allowing for bicarbonate reabsorption into the blood.

V-ATPase structure

The yeast V-ATPase is the best characterized. There are at least 13 subunits identified to form a functional V-ATPase complex, which consists of two domains. The subunits belong to either the Vo domain (membrane associated subunits, lower case letters on the figure), or the V1 domain (peripherally associated subunits, upper case letters on the figure).

The V1 includes 8 subunits, A-H, with three copies of the A and B subunits, one or two copies of E, and two copies of subunit G. The V1 domain contains tissue specific subunit isoforms including B, C, E, and G. Mutations to the B1 isoform result in the human disease distal renal tubular acidosis and sensorineural deafness.

The Vo domain contains 6 different subunits, a, d, c, c', c" and e, with possibly four copies of c and one copy of the remaining subunits. The mammalian Vo domain contains tissue specific isoforms for subunits a and d, while yeast V-ATPase contains two organelle specific subunit isoforms of a, Vph1p and Stv1p. Mutations to the a3 isoform result in the human disease infantile malignant osteopetrosis, and mutations to the a4 isoform result in distal renal tubular acidosis, in some cases with sensorineural deafness.

The V1 domain is responsible for ATP hydrolysis whereas the Vo domain is responsible for proton translocation. ATP hydrolysis at the catalytic nucleotide binding sites on subunit A drives rotation of a central stalk composed of subunits D and F, which in turn drives rotation of a barrel of c subunits relative to the a subunit. The amino-terminal of a (NT-a) along with subunits C, E, G and H compose the peripheral stalk. The carboxy-terminal of subunit a (CT-a) is held fixed relative to the A3B3 head by this peripheral stalk. Movement of the barrel of c subunits past the a subunit is thought to drive proton transport across the membrane. A stoicheometry of two protons translocated for each ATP hydrolyzed has been proposed by (Johnson, 1982).

In addition to the structural subunits of yeast V-ATPase, associated proteins have been identified that are necessary for assembly. These associated proteins are essential for Vo domain assembly and are termed Vma12p, Vma21p and Vma22p. Two of the three proteins, Vma12p and Vma22p form a complex that binds transiently to Vph1p (subunit a) to aid its assembly and maturation. Vma21p coordinates assembly of the Vo subunits as well as escorting the Vo domain into vesicles for transport to the Golgi.

V-ATPase assembly

Yeast V-ATPases fail to assemble when any of the genes that encode subunits are deleted except for subunits H and c". Without subunit H, the assembled V-ATPase is not active and the loss of the c" subunit results in uncoupling of enzymatic activity.

The precise mechanisms by which V-ATPases assembly are still controversial with evidence suggesting two different possibilities. Mutational analysis and in vitro assays have shown that preassembled Vo and V1 domains can combine to form one complex in a process called independent assembly. Support for independent assembly includes the findings that the assembled Vo domain can be found at the vacuole in the absence of the V1 domain, whereas free V1 domains can be found in the cytoplasm and not at the vacuole. In contrast, in vivo pulse-chase experiments have revealed early interactions between Vo and V1 subunits, specifically the a and B subunits, suggesting that subunits are added in a step-wise fashion to form a single complex in a concerted assembly process.

Regulation of V-ATPase activity

In vivo regulation of V-ATPase activity is accomplished by reversible dissociation of the V1 domain from the Vo domain. After inital assembly, both the insect Manduca sexta and yeast V-ATPases can reversibly disassemble into free Vo and V1 domains after a 2-5 min deprivation of glucose. Reversible disassembly may be a general mechanism of regulating V-ATPase activity since it exists in yeast and insects. Reassembly is proposed to be aided by a complex termed RAVE (regulator of H+-ATPase of vacuolar and endosomal membranes). Interestingly, dissasembly and reassembly of V-ATPases does not require new protein synthesis but does need an intact microtubular network.

Human diseases

Osteopetrosis

Osteopetrosis is generic name that represents a group of heritable conditions in which there is a defect in osteoclastic bone resorption. Both dominant and recessive osteopetroses occur in humans. Autosomal dominant osteopetrosis shows mild symptoms in adults who experience frequent bone fractures due to brittle bones. A form of osteopetrosis that is clinically more severe is termed autosomal recessive infantile malignant osteopetrosis. Three genes have been identified which are responsible for recessive osteopetrosis in humans. Interestingly, they are all directly involved in the proton generation and secretion pathways that are essential for bone resorption. One gene is carbonic anhydrase II (CAII) that when mutated causes osteopetrosis with renal tubular acidosis. Mutations to the chloride channel ClC7 gene also lead to both dominant and recessive osteopetrosis. Approximately 50% of patients with recessive infantile malignant osteopetrosis have mutations to the a3 subunit isoform of V-ATPase. In humans, 26 mutations have been identified in V-ATPase subunit isoform a3, found in osteoclasts, that result in the bone disease autosomal recessive osteopetrosis.

Distal renal tubular acidosis (dRTA)

The importance of V-ATPase activity in renal proton secretion is highlighted by the inherited disease distal renal tubule acidosis. In all cases, renal tubular acidosis results from a failure of the normal renal mechanisms that regulate systemic pH. There are four types of renal tubular acidosis. Type 1 is Distal renal tubular acidosis and results from a failure of the distal nephron to acidify the urine below pH 5. Some patients with recessive dRTA also have sensorineural hearing loss. Inheritance of this type of RTA results from mutations to either V-ATPase subunit isoforms B1 or a4, or mutations to the AE1 Cl-/HCO3- exchanger. Twelve different mutations to V-ATPase isoform B1 and twenty-four different mutations in a4 lead to dRTA. Reverse transcription polymerase chain reaction studies have shown expression of the a4 subunit in the intercalated cell of the kidney and in the cochlea. dRTA caused by mutations in the a4 subunit gene in some cases can be associated with deafness.

Nomenclature

The term Vo has a lowercase letter "o" (not the number "zero") in subscript. The "o" stands for oligomycin.

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