Peptidoglycan

Peptidoglycan, also known as murein, is a polymer consisting of sugars and amino acids that forms a homogeneous layer outside the plasma membrane of eubacteria. Archaea have a similar layer of pseudopeptidoglycan. Peptidoglycan serves a structural role in the bacterial cell wall, giving the wall shape and structural strength, as well as counteracting the osmotic pressure of the cytoplasm. Peptidoglycan is also involved in binary fission during bacterial cell reproduction.

The peptidoglycan layer is substantially thicker in Gram-positive bacteria (20 to 80 nm) than in Gram-negative bacteria (7 to 8 nm), with the attachment of the S-layer. Peptidoglycan forms around 90% of the dry weight of Gram-positive bacteria but only 10% of Gram-negative strains.

Antibiotic inhibition

Antibacterial drugs such as penicillin target the peptidoglycan layer by interfering with its formation, specifically the crosslinking enzyme transpeptidase. Mutations in the transpeptidase enzyme (also known as penicillin binding protein or PBP) which lead to reduced interactions between an antibiotic and the bacterial PBPs are a significant cause of emerging antibiotic resistance.

Structure

The peptidoglycan layer in the bacterial cell wall is a crystal lattice structure formed from linear chains of two alternating amino sugars, namely N-acetyl glucosamine (GlcNAc) and N-acetyl muramic acid (MurNAc). Each MurNAc is attached to a short (4 to 5 residue) amino acid chain. Cross-linking between amino acids in different linear amino sugar chains by an enzyme known as transpeptidase result in a 3-dimensional structure that is strong and rigid. The specific amino acid sequence and molecular structure vary with the bacterial species.

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